| Round 1 |
| Saturday, 27 September 2025 |
| 9 | Northwich 2nd XV | Home Walkover | Liverpool Uni Vets 1 |
| Saturday, 4 October 2025 |
| 1 | Oxton Parkonians | 34 | 12 | Birchfield |
| 2 | Chester 3rd XV | 12 | 27 | Caldy 3rd XV |
| 6 | Trafford MV 2nd XV | Home Walkover | Warrington 2 |
| 7 | Preston Grasshoppers 4th XV | 0 | 46 | Bury 2nd XV |
| 8 | Port Sunlight | 38 | 12 | Sefton 2nd XV |
| 10 | North Manchester | 10 | 41 | Garstang Blues (2nd XV) |
| 11 | Moore | 78 | 0 | Heaton Moor 2nd XV |
| 12 | Blackburn RUFC Vikings | 72 | 7 | Eccles 3rd XV |
| 13 | Bolton 2nd XV | Home Walkover | Newton le Willows 1 |
| 14 | Wilmslow 2nd XV | 47 | 12 | Winnington Park 2nd XV |
| 15 | Capenhurst | 20 | 14 | New Brighton 2nd XV |
| 16 | Macclesfield 3rd XV | Away Walkover | Stockport 3rd XV |
| Saturday, 18 October 2025 |
| 4 | Liverpool University Veterinarians | 26 | 5 | Glossop 2nd XV |
| Saturday, 25 October 2025 |
| 3 | Warrington 2nd XV | 72 | 12 | Vale of Lune 3rd XV |
| 5 | Newton-le-Willows | v | Crosby St Mary's |
| Round 2 |
| Saturday, 1 November 2025 |
| Warrington 2nd XV | Home Walkover | Liverpool University Veterinarians |
| Newton-le-Willows | 37 | 5 | Trafford MV 2nd XV |
| Northwich 2nd XV | 40 | 14 | Garstang Blues (2nd XV) |
| Moore | 33 | 33 | Blackburn RUFC Vikings |
| Bolton 2nd XV | Away Walkover | Wilmslow 2nd XV |
| Capenhurst | 23 | 33 | Stockport 3rd XV |
| Saturday, 15 November 2025 |
| Bury 2nd XV | 60 | 10 | Port Sunlight |
| Saturday, 10 January 2026 |
| Oxton Parkonians | v | Caldy 3rd XV |
| Round 3 |
| Saturday, 7 February 2026 |
| Warrington 2nd XV | v | Northwich 2nd XV |
| Newton-le-Willows | v | Blackburn RUFC Vikings |
| Bury 2nd XV | v | TBC |
| Wilmslow 2nd XV | v | Stockport 3rd XV |
| Round 4 |
| Saturday, 14 March 2026 |
| TBC | v | TBC |
| TBC | v | TBC |
| Round 5 |
| Saturday, 2 May 2026 |
| TBC | v | TBC |